No, Plo Koon does not appear in Star Wars: The Acolyte. However, a member of his species, the Kel Dor, does appear in episode 4 as part of the Jedi expedition to Khofar.
Plo Koon would have shown a fatherly love and care ... in Obi-Wan’s stead after his death. Out of all of Anakin’s potential ...