The hierarchical shotgun sequencing strategy involves several steps. First, a library is created by fragmenting the target genome and cloning it into vectors. Next, the genomic DNA fragments are ...
2001); this approach was similar to the hierarchical shotgun approach used by the IHGSC ... the DNA template they were interested in sequencing with DNA polymerase, a single-stranded DNA primer ...
The public Human Genome Project used a hierarchical shotgun (HS) approach, whereas Celera Genomics chose a whole-genome shotgun (WGS) strategy. In the Early Edition of the Proceedings of the National ...
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